Recognition of Isomers by Mass Spectrometry Using the Kinetic Method

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  • V. Ranc Department of Analytical Chemistry, Faculty of Science, Palacký University, Olomouc, Czech Republic
  • P. Fryčák Department of Analytical Chemistry, Faculty of Science, Palacký University, Olomouc, Czech Republic
  • L. Müller Department of Analytical Chemistry, Faculty of Science, Palacký University, Olomouc, Czech Republic
  • P. Bednář Department of Analytical Chemistry, Faculty of Science, Palacký University, Olomouc, Czech Republic
  • K. Lemr Department of Analytical Chemistry, Faculty of Science, Palacký University, Olomouc, Czech Republic

Abstrakt

The principle of the Cooks´kinetic method and its application to analysis of isomeric mixtures are described. The kinetic method based on mass spectrometric measurements is applied to discrimination of isomers of various types of compounds (amino acids, peptides, sugars, pharmaceuticals, etc.). So far this method has been used in static systems (with constant analyte concentrations in the course of analytical run). A modification for dynamic systems (flow injection analysis, HPLC) is presented. The accuracy of determination in both static and dynamic systems can worsen due to different ratios of the isomers in liquid and gas phases. A procedure for correction of this phenomenon is also demonstrated.

Publikováno

15.04.2006

Jak citovat

Ranc, V., Fryčák, P., Müller, L., Bednář, P., & Lemr, K. (2006). Recognition of Isomers by Mass Spectrometry Using the Kinetic Method. Chemické Listy, 100(3). Získáno z http://ww-w.chemicke-listy.cz/ojs3/index.php/chemicke-listy/article/view/1944

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