Štúdium molten globulárneho stavu cytochrómu c pomocou viskozimetrie
Klíčová slova:molten globulárny, cytochróm c, viskozimeter
Combination of viscometry and CD spectrometry made it possible to suggest the existence of a new conformational state of cytochrome c at high temperatures and ionic strengths. The molten globule state of cytochrome c was found at acidic pH in the presence of high concentrations of a salt at 20 °C. A low-cooperative conformational change of the cytochrome c to the unfolded state was observed with increasing temperature. Viscometry is a suitable tool for studies of similar processes in biomacromolecules.