Validácia stanovenia kyseliny trans,trans-mukonovej ako biomarkera expozície benzénu metódou HPLC

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  • I. Bajusová Klinika pracovného lekárstva a klinickej toxikológie, Univerzitná nemocnica L. Pasteura Košice, Košice
  • Ľ. Legáth Klinika pracovného lekárstva a klinickej toxikológie, Lekárska fakulta, Univerzita P. J. Šafárika Košice, Košice
  • T. Gondová Katedra analytickej chémie, Prírodovedecká fakulta, Univerzita P. J. Šafárika Košice, Košice
  • Z. Vargová Katedra anorganickej chémie, Prírodovedecká fakulta, Univerzita P. J. Šafárika Košice, Košice

Klíčová slova:

biomarker, benzén, kyselina trans,trans-mukonová, HPLC, moč

Abstrakt

Determination of phenol in urine is a widely used method. However, it may provide false positive results due to possible presence of aromatic amino acids, which can also produce phenol in urine. Trans,trans-muconic acid (MA) is a non-phenolic metabolite of benzene which appears in urine. The present review deals with determination of MA by RP-HPLC with UV detection. MA was isolated from urine by solid phase extraction (SPE). The mean recovery was 96-103 %. The detection and quantification limits were 0.057 mg l–1 and 0.189 mg l–1, respectively. This method can be used to determine, control and monitor the level of MA in urine of workers, who are overexposed to benzene. The obtained data were compared with those of the non-exposed control group. The results suggest that MA could be one of the validated benzene bio­markers in risk assessment.

Stahování

Publikováno

15.04.2012

Jak citovat

Bajusová, I., Legáth, Ľ., Gondová, T., & Vargová, Z. (2012). Validácia stanovenia kyseliny trans,trans-mukonovej ako biomarkera expozície benzénu metódou HPLC. Chemické Listy, 106(4), 293–298. Získáno z http://ww-w.chemicke-listy.cz/ojs3/index.php/chemicke-listy/article/view/980

Číslo

Vol 106 No 4 (2012)

Sekce

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