Immunochemical Detections of Genus Cronobacter

Authors

  • Martina Blažková Department of Biochemistry and Microbiology, Institute of Chemical Technology, Prague
  • Barbora Javůrková Department of Biochemistry and Microbiology, Institute of Chemical Technology, Prague
  • Ladislav Fukal Department of Biochemistry and Microbiology, Institute of Chemical Technology, Prague
  • Pavel Rauch Department of Biochemistry and Microbiology, Institute of Chemical Technology, Prague

Keywords:

Cronobacter, Enterobacter sakazakii, ELISA, enzyme immunoassay, LFIA, immunochromatography

Abstract

Both immunochemical methods, sandwich ELISA and immunochromatographic detection, for detection of genus Cronobacter, have been developed. Rabbit polyclonal antibodies against dead cells of Cronobacter sakazakii Cb03 (ATCC 29544) were used. In tests of bacteria cultures from microbial collection, the ELISA distinguished between all assessed Cronobacter and Enterobacter strains. The immunochromatographic technique provided false negative results for 20 % of Cronobacter strains, and 40 % false positive results for Enterobacter strains.

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Published

2011-03-15

How to Cite

Blažková, M., Javůrková, B., Fukal, L., & Rauch, P. (2011). Immunochemical Detections of Genus Cronobacter. Chemické Listy, 105(3), 193–198. Retrieved from http://ww-w.chemicke-listy.cz/ojs3/index.php/chemicke-listy/article/view/1168

Issue

Vol. 105 No. 3 (2011)

Section

Articles