Analysis of Antioxidant Activity of Polyphenolic Compounds of Lichens and Liverworts Using Thin Layer Chromatography with Detection by 2,2-Diphenyl-1-picrylhydrazyl
Keywords:
antioxidant activity, flavonoids, lichen acids, marchantin, TLC-DPPH methodAbstract
This paper deals with the analysis of antioxidant activities of polyphenolic compounds by TLC-DPPH method. Polyphenolic compounds are represented by lichen acids (depsides, benzyldepsides, depsidones, dibenzofuranes and antraquinones) and dihydrostilbenoids, macrocyclic dimeric dihydrostilbenoids, flavonoids occurring in liverworts. The highest antioxidant activity was observed in the case of 3-hydroxyphysodic acid from Hypogymnia physodes, marchantin A and flavonoids from Marchantia polymorpha. The assessment of the TLC-DPPH method shows that it is suitable for an easy and fast analysis of the antioxidant potential of the polyphenolic compounds occurring in the thalus of lichens and liverworts. The filter paper modification of this method is also discussed.