Quantitative Analysis by Mass Spectrometry

Abstract

The present paper is an overview of basic concepts important for quantitative mass spectrometry, such as limit of detection, sensitivity, signal-to-noise ratio, method detection limit, instrument detection limit, limit of quantification, and calibration curve model. The topics are mainly covered from the perspective of small molecule LC-MS. The text includes detailed discussion of different approaches that can be used to define limit of quantification, from traditional signal-to-noise ratio and standard deviation definition to more recent definitions utilizing accuracy and precision. Usage of internal standards and certified materials is also briefly described.