Determination of Alloxan in Flour by Capillary Electrophoresis with Tandem Mass Spectrometry

Authors

  • J. Horská Department of Physics, Chemistry and Vocational Education, Faculty of Education, Masaryk University, Brno
  • A. Obšívačová Department of Analytical Chemistry, Faculty of Science, Palacky University, Olomouc
  • K. Vítková University Hospital Ostrava, Ostrava
  • J. Jurčíková University Hospital Ostrava, Ostrava
  • V. Procházka University Hospital Ostrava, Ostrava | University of Ostrava, Faculty of Medicine, Ostrava
  • J. Petr Department of Analytical Chemistry, Faculty of Science, Palacky University, Olomouc

Keywords:

alloxan, diabetes, mass spectrometry, capillary electrophoresis

Abstract

We have developed a fast and efficient method for the determination of alloxan in flour samples by capillary electrophoresis with tandem mass spectrometry without derivatization step. Mass spectra of alloxan were studied in detail providing characteristic signals at m/z 159, 141 and 115. The separation lasted 8 min under the optimum conditions using 50 mM ammonium acetate pH 9.5 as the background electrolyte and uric acid as the internal standard. Five flour samples including bleached flour from the USA were analyzed and alloxan was not detected in any of these samples.

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Published

2020-07-15

How to Cite

Horská, J., Obšívačová, A., Vítková, K., Jurčíková, J., Procházka, V., & Petr, J. (2020). Determination of Alloxan in Flour by Capillary Electrophoresis with Tandem Mass Spectrometry. Chemické Listy, 114(7), 454–457. Retrieved from http://ww-w.chemicke-listy.cz/ojs3/index.php/chemicke-listy/article/view/3639

Issue

Vol. 114 No. 7 (2020)

Section

Articles

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