Characteristics of Barley Malt by HPLC

Authors

  • K. Benešová Malting Institute, Brno
  • I. Hartman Malting Institute, Brno
  • S. Běláková Malting Institute, Brno
  • R. Mikulíková Malting Institute, Brno

Keywords:

malt, tocopherols, tocotrienols, carotenoids, ergosterol, spring barley

Abstract

A fast and sensitive HPLC for simultaneous determination of vitamin E, carotenoids and ergosterol was elaborated. The method includes alkaline hydrolysis, extraction with an organic solvent, chromatographic separation and detection with a photodiode-array and fluorescence detector. The analytes were separated by HPLC using acetonitrile as a mobile phase. The method was validated and applied to malt analysis.

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Published

2014-10-15

How to Cite

Benešová, K., Hartman, I., Běláková, S., & Mikulíková, R. (2014). Characteristics of Barley Malt by HPLC. Chemické Listy, 108(10), 961–966. Retrieved from http://ww-w.chemicke-listy.cz/ojs3/index.php/chemicke-listy/article/view/451

Issue

Vol. 108 No. 10 (2014)

Section

Articles

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